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Welch Ultisil® HILIC Amphion II is a newly developed HILIC column, using amphion-bonded silica as packing material. It applies to the separation of most polar compounds, using acetonitrile or Water other than ion-pairing reagents as mobile phase. The Amphion, containing both Positive Charge Centre and Negative Charge Centre, brings high retention for acid and alkaline compounds through ion-exchange mechanism. Compared with common HILIC packing materials like silica and amino groups, the Amphion-bonded packing material provides better reproducibility and stability.