Hamilton PRP-X600 (WAX for Proteins & DNA Oligomers)

PRP-X600 is a superficially porous weak-base anion exchange support that separates DNA oligomers according to negative charge. The unique porosity provides fast separation with better sample capacity than non-porous supports. The superficially porous properties shorten the diffusion path of the analyte, resulting in sharp sample bands. The PRP-X600, a hydrophilic methacrylate-based polymer, improves sample recovery due to minimized hydrophobic interactions. Because the PRP-X600 is a weak anion exchange (WAX) resin, the exchange capacity of the resin is pH dependent. Lowering the pH will reduce the binding of proteins, reducing the run time for a complete separation. Change the mobile phase composition to alter the retention of DNA oligomers. Rapid gradient changes typically lower column efficiency; however, biomolecules run in this fashion on the PRP-X500 show very favorable separation efficiency with much shorter run times.