Agilent Bio Mab - 3µm

Thorough characterization of monoclonal antibodies includes the identification and monitoring of acidic and basic isoforms. The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies. Bio MAb columns are available in 1.7μm, 3μm, 5μm, and 10μm sizes, providing higher resolution with the smaller particles. The Bio MAb column features a highly uniform, densely packed, weak cation exchange layer. This layer of the Bio MAb column is chemically bonded to the hydrophilic, polymeric coating of the Bio MAb column.

  • A packing support composed of a rigid, spherical, highly cross-linked polystyrene divinylbenzene (PS/DVB) non-porous bead
  • Particles grafted with a hydrophilic, polymeric layer, virtually eliminating non-specific binding of antibody proteins
  • A different process is used to layer the weak cation-exchange phase to the particle making it a higher density than the Agilent Bio WCX column particles
  • Specifically designed for the separation of charge isoforms of monoclonal antibodies

Application Note - Analysis of Intact and C-terminal Digested IgG1 on an Agilent Bio MAb 5µm Column

Application Note - pH Gradient Elution for Improved Separation of Monoclonal Antibody Charge Variants


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AT-5190-2403 Agilent Bio MAb 3µm, 4.6 x 50mm (Stainless Steel), ea. price on request
AT-5190-2404 Agilent Bio MAb 3µm, Guard 4.0 x 10mm (Stainless Steel), ea. price on request