TweenTrap Series (Trap for Surfactants)

The Sepax TweenTrap Column is specifically designed for trapping surfactants, such as Polysorbate 80/20 (PS 80/20, Tween 80/20), in aqueous samples. The packing support is composed of rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) beads with a particle size of 5μm and a proprietary surface coating is covalently bonded onto the PS/DVB support. TweenTrap columns can be run in tandem with SEC columns in aqueous samples, and are specifically designed to trap and remove surfactants, such as Polysorbate 80/20, which can interfere with quantitative protein SEC analysis.

• Run the TweenTrap column with SEC column in aqueous based mobile phases, without the addition of organic solvents.
• Perform CIP wash every 20 injections, when the concentration of tween is 0.01% in sample
• Disconnect the TweenTrap column from the SEC column before the cleaning
• Trapped surfactants can be cleaned with one of two methods

TweenTrap Flyer

TweenTrap User Manual