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HILIC (Hydrophilic Interaction Liquid Chromatography) is a separation mode achieved through the partitioning of polar solutes from high concentration, water-miscible, organic mobile phase into hydrophilic surface environment. Welch Ultisil HILIC Amide column is a special column designed for HILIC mode. As amide group has strong hydrophilicity, stability and electrically neutral, Ultisil® Amide has longer life, better separation repeatability and peak shape than NH2 phase does.