TweenTrap Series (Trap for Surfactants)
The Sepax TweenTrap Column is specifically designed for trapping surfactants, such as Polysorbate 80/20 (PS 80/20, Tween 80/20), in aqueous samples. The packing support is composed of rigid, spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) beads with a particle size of 5μm and a proprietary surface coating is covalently bonded onto the PS/DVB support. TweenTrap columns can be run in tandem with SEC columns in aqueous samples, and are specifically designed to trap and remove surfactants, such as Polysorbate 80/20, which can interfere with quantitative protein SEC analysis.
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Run the TweenTrap column with SEC column in aqueous based mobile phases, without the addition of organic solvents.
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Perform CIP wash every 20 injections, when the concentration of tween is 0.01% in sample
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Disconnect the TweenTrap column from the SEC column before the cleaning
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Trapped surfactants can be cleaned with one of two methods