Regis Coelenterazine

Coelenterazine is the substrate that illuminates many invertebrate marine animals such as the luminescent Aequorea jellyfish. Like firefly luciferin, coelenterazine can be used in:

• Gene reporter assays
• Whole animal assays
• Biochemical assays (i.e., ELISA and Bioluminescence Resonance Energy Transfer [BRET])
• Live-cell assays for the detection of calcium ions

When coelenterazine combines with luciferase, oxygen, and calcium ions, it produces oxy-coelenterazine and light. This allows coelenterazine to be used as a detection reagent for luciferase, oxygen, or calcium. Unlike fluorescence, the detection of bioluminescence in diagnostic tests can be performed in the dark thereby eliminating background noise.

Regis Bioluminescence Specialty Reagents